{"id":59160,"date":"2026-07-02T15:37:39","date_gmt":"2026-07-02T13:37:39","guid":{"rendered":"https:\/\/botiss.com\/?post_type=product&p=59160"},"modified":"2026-08-28T14:31:40","modified_gmt":"2026-08-28T12:31:40","slug":"early-osteogenic-and-stromal-marker-responses-to-a-hyaluronic-acid-coated-xenogeneic-bone-graft-exploratory-in-vitro-analysis-cerabonehya","status":"publish","type":"product","link":"https:\/\/botiss.com\/de\/produkt\/early-osteogenic-and-stromal-marker-responses-to-a-hyaluronic-acid-coated-xenogeneic-bone-graft-exploratory-in-vitro-analysis-cerabonehya\/","title":{"rendered":"Early Osteogenic and Stromal Marker Responses to a Hyaluronic Acid-Coated Xenogeneic Bone Graft \u2013 Exploratory In Vitro Analysis"},"content":{"rendered":"

Dent. J. 2026, 14(5), 290; <\/span><\/strong><\/a>https:\/\/doi.org\/10.3390\/dj14050290<\/a><\/strong><\/p>\n

Original title: Early Osteogenic and Stromal Marker Responses of Osteoblast-like and Bone-Marrow Stromal Cell Lines to a Hyaluronic Acid-Coated Xenogeneic Bone Graft: An Exploratory In Vitro Analysis \u2013 Yaniv Mayer, Hia Abu Sada, Hadar Zigdon Giladi, Eran Gabay, Ofri Doppelt-Flikshtain, Ofir Ginesin<\/p>\n

In this exploratory in vitro study, osteoblast-like cells exposed to cerabone\u00ae<\/sup> +HyA<\/a> showed a 2.61-fold increase in RUNX2 expression compared with untreated cells, together with increased COL1A1 expression. These findings indicate an upregulation of early osteogenic markers under the tested conditions. After 48 hours, the cerabone\u00ae<\/sup> +HyA<\/a> group showed a numerically higher mean metabolic activity; however, the differences were not statistically significant. All three xenografts, whether HA-coated or uncoated, reduced the expression of fibrotic markers in bone marrow stromal cells to a similar extent.<\/p>\n<\/div>

AIM
\n<\/strong>To investigate whether coating a xenogeneic bone graft with hyaluronic acid influences early osteogenic and fibrotic responses in vitro<\/strong> by comparing an HA-coated xenograft with two uncoated bovine bone graft materials.<\/p>\n<\/div>

STUDY DESIGN
\n<\/strong>Three commercially available xenografts were evaluated: cerabone\u00ae<\/sup> +HyA<\/strong><\/a> (HA-coated), cerabone\u00ae<\/sup><\/a><\/strong> (its uncoated equivalent from the same manufacturer), and Bio-Oss\u00ae<\/sup><\/strong> (an independent bovine bone substitute manufactured using a different process).<\/p>\n

Human osteoblast-like cells (U2OS) and bone marrow stromal cells (HS5) were cultured in the presence of extracts from each material. Cell metabolic activity was assessed after 24 and 48 hours using an XTT assay. Cell adhesion to the graft surface was examined by fluorescence microscopy, while the osteogenic markers RUNX2<\/strong>and COL1A1<\/strong>, together with the fibrotic markers COL3A1<\/strong> and TGF-\u03b23<\/strong>, were quantified using real-time PCR. Each xenograft group was compared with untreated control cells.<\/p>\n

The cerabone\u00ae<\/sup> +HyA<\/strong><\/a> extract was tested at half the concentration of the extracts from the two comparator materials.<\/p>\n<\/div>

RESULTS
\n<\/strong>Metabolic activity (XTT):
\n<\/strong>Cell metabolic activity was comparable across all groups after 24 hours. At 48 hours, cerabone\u00ae<\/sup> +HyA<\/strong><\/a>\u00a0showed a numerically higher mean value (0.538 \u00b1 0.056), followed by cerabone\u00ae<\/sup><\/a> <\/strong>(0.450 \u00b1 0.120) and Bio-Oss\u00ae<\/sup><\/strong> (0.439 \u00b1 0.073). However, after adjustment for multiple comparisons, these differences were not statistically significant.<\/p>\n

Osteogenic markers:<\/strong>
\nCells exposed to cerabone\u00ae<\/sup> +HyA<\/strong><\/a> exhibited a 2.61-fold increase in RUNX2 expression<\/strong>compared with untreated controls (p = 0.01). Together with the observed increase in COL1A1 expression, this finding suggests that exposure to cerabone\u00ae<\/sup> +HyA<\/strong><\/a> was associated with increased early osteogenic marker expression under the tested conditions. The study did not establish statistically significant superiority over the other xenografts.<\/p>\n

Cell adhesion:
\n<\/strong>Cells adhered to all three graft surfaces after 48 hours. As adhesion was assessed qualitatively rather than quantitatively, no comparison of adhesion performance between the materials could be made.<\/p>\n

Fibrotic markers:
\n<\/strong>Expression of COL3A1<\/strong> and TGF-\u03b23<\/strong> decreased by approximately 92.7%<\/strong> and 92.1%<\/strong>, respectively, in stromal cells exposed to all three xenografts (both p = 0.001), with no meaningful differences between the materials.<\/p>\n<\/div>

CONCLUSION
\n<\/strong>In this exploratory in vitro model, exposure to cerabone\u00ae<\/sup> +HyA<\/strong><\/a> was associated with increased expression of the early osteogenic markers RUNX2<\/strong> and COL1A1<\/strong> compared with untreated controls. Metabolic activity was numerically higher in the cerabone\u00ae<\/sup> +HyA<\/strong><\/a> group at 48 hours, but the differences between groups were not statistically significant. All three xenografts showed a similar reduction in stromal marker expression. These exploratory findings suggest a potential role for cerabone\u00ae<\/sup> +HyA<\/strong><\/a> in supporting early osteogenic responses under the tested in vitro conditions and warrant further investigation.<\/p>\n

However, the study has several limitations. It used established cell lines rather than primary human cells, evaluated only a limited number of markers at a single early time point, and tested the cerabone\u00ae<\/sup> +HyA<\/strong><\/a> extract at a lower concentration than the comparator materials. Further studies using primary human cells, equivalent extract concentrations, animal models, and clinical investigations are needed to confirm these findings.<\/p>\n<\/div><\/div><\/div><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"featured_media":59162,"template":"","meta":{"_acf_changed":false,"content-type":"","footnotes":""},"product_brand":[],"product_cat":[327],"product_tag":[273,238],"class_list":["post-59160","product","type-product","status-publish","has-post-thumbnail","product_cat-studies","product_tag-cerabone","product_tag-cerabone-hya","first","instock","product-type-simple"],"acf":[],"yoast_head":"\nEarly Osteogenic and Stromal Marker Responses to a Hyaluronic Acid-Coated Xenogeneic Bone Graft \u2013 Exploratory In Vitro Analysis - botiss biomaterials GmbH<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/botiss.com\/de\/produkt\/early-osteogenic-and-stromal-marker-responses-to-a-hyaluronic-acid-coated-xenogeneic-bone-graft-exploratory-in-vitro-analysis-cerabonehya\/\" \/>\n<meta property=\"og:locale\" content=\"de_DE\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Early Osteogenic and Stromal Marker Responses to a Hyaluronic Acid-Coated Xenogeneic Bone Graft \u2013 Exploratory In Vitro Analysis\" \/>\n<meta property=\"og:url\" content=\"https:\/\/botiss.com\/de\/produkt\/early-osteogenic-and-stromal-marker-responses-to-a-hyaluronic-acid-coated-xenogeneic-bone-graft-exploratory-in-vitro-analysis-cerabonehya\/\" \/>\n<meta property=\"og:site_name\" content=\"botiss biomaterials GmbH\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/botissdental\" \/>\n<meta property=\"article:modified_time\" content=\"2026-08-28T12:31:40+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/botiss.com\/wp-content\/uploads\/2026\/07\/Study_Mayer_CAMPUS.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1200\" \/>\n\t<meta property=\"og:image:height\" content=\"800\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:site\" content=\"@BotissB\" \/>\n<meta name=\"twitter:label1\" content=\"Gesch\u00e4tzte Lesezeit\" \/>\n\t<meta name=\"twitter:data1\" content=\"3\u00a0Minuten\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/botiss.com\\\/de\\\/produkt\\\/early-osteogenic-and-stromal-marker-responses-to-a-hyaluronic-acid-coated-xenogeneic-bone-graft-exploratory-in-vitro-analysis-cerabonehya\\\/\",\"url\":\"https:\\\/\\\/botiss.com\\\/de\\\/produkt\\\/early-osteogenic-and-stromal-marker-responses-to-a-hyaluronic-acid-coated-xenogeneic-bone-graft-exploratory-in-vitro-analysis-cerabonehya\\\/\",\"name\":\"Early Osteogenic and Stromal Marker Responses to a Hyaluronic Acid-Coated Xenogeneic Bone Graft \u2013 Exploratory In Vitro Analysis - 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