Histological and histomorphometrical analysis on the Ossification of Jason® membrane – an animal study
Alkildani S, Ren Y, Liu L, Rimashevskiy D, Schnettler R, Radenković M, Najman S, Stojanović S, Jung O, Barbeck M. Int J Mol Sci. 2023 Apr 6;24(7):6833.

Original Title: Analyses of the Cellular Interactions between the Ossification of Collagen-Based Barrier Membranes and the Underlying Bone Defects


The goal of the study was to investigate the effect of Jason® membrane on the osseous regeneration of the covered bone defects. It was further the aim to analyze and quantify the ossifying process of the pericardium membrane, i.e. the transition of the membrane into vital bone.


Surgically created rat calvaria defects were filled with cerabone® plus or were left untreated without bone graft. In both groups the defects were covered with Jason® membrane and the sites were sutured. Histological and histomorphometrical analyses of the augmented sites including the membrane areas were performed two-, eight- and 16-weeks post-augmentation. The main outcome parameter was the bone regeneration in proximity of the membrane both from a qualitative and quantitative standpoint.


  • Jason® membrane gradually resorbed while it was completely transformed into bone tissue (Fig. 1)
  • Ossified Jason® membrane formed an independent bony layer above the actual bone defects
  • Higher degree of membrane ossification in the cerabone® plus grafted sites at 16 weeks post-surgery (p < 0.01)
  • Comparable vessel densities and vascularization percentages in both groups

Fig. 1 Ossification of Jason® membrane at eight weeks after implantation. Significant percentages (p < 0.05) of the membranes in both groups were ossified, demonstrating its transition into bone. (Movat’s Pentachrome-staining, 130× magnification)


Jason® membrane stimulates osteogenesis as it completely remodeled into newly formed bone independent of the bony regeneration of the defects it covered.

The ossification of Jason® membrane and the associated generation of a “bony shield” may support bone regeneration of the underlying bone defect by means of additional bone ingrowth and due to the efficient protection of the underlying defect area.

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