A high vitamin D level for
dental treatment success

In medicine, there is clear evidence for the importance of vitamin D in clinical treatment.

Scientific studies have shown that having a sufficient vitamin D level has a significant influence on the success of dental treatments; from prophylaxis to caries and implantology.

botissCARE – dental prevention re-thought


It is essential for your treatment success to know the vitamin D status of your patients. Assess your patients in your practice using an immediate chairside test from botissCARE. Plan your therapy tailored to the individual needs of your patients.

This creates a basis for evidence-based improvement in their treatment outcomes and success rates, in which the patient also participates.


A high vitamin D level has a significant impact on improving dental treatment success.

With botissCARE solutions, you integrate a toolbox for testing, quantitative analysis, and optimization of your patients’ vitamin D3+K2 balance into your treatment workflow – immediately and chairside in your practice.

We support you in the smooth integration of vitamin D into your practice routine and additionally provide you with tools for proper patient communication.

For further questions and answers, read our FAQs here


Do you have your practice in Germany? Then order directly here:

You will be redirected to our sales partner Straumann.

If your practice is located in Austria, please contact our product management. If your practice is located outside of Germany and Austria, we will be happy to inform you when other countries are added. To stay up to date, please register here for our newsletter.

botissCARE stands for innovation and scientific expertise in bone and soft tissue regeneration.

The perfect partner for dentists who strive for the best results for their patients.


botissCARE Rapi-D
Quantitative vitamin D test

botissCARE Cube Reader
Reader for in vitro diagnostics

botissCARE D3+K2
10 ml Spray



botissCARE offers you coordinated solutions for immediate, quantitative determination and optimization of vitamin D levels. You receive all components first-hand from your reliable partner with scientific expertise.

15 MinuteS

The reliable result of our minimally invasive chairside test is available to you after only 15 minutes. This provides you with a reliable basis for decision-making for further consultation with your patient.


By regularly checking and adjusting the vitamin D level of your patients, you reduce the risk of complications such as unpredictable implant loss and thus increase planning reliability and patient satisfaction.



“We are not just mechanics, we are doctors of the oral cavity. And as such, if we’re going to do complex surgery, let’s measure vitamin D, let’s supplement, and let’s just add that extra measure to our patients, allowing us to not just be dentists, but also healers.”
Dr. Miguel Stanley

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“In my practice, the botissCARE system represents a valuable new building block in my implantology and periodontology treatment concepts. Through the simple chair side application, my patients can very quickly get an insight into their current vitamin D level. Individual, rapid decision-making on the necessary preventive care becomes possible.”
Dr. Jochen Tunkel

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“For me, the botissCARE offer fills a gap I have been aware of for some time between professional dentistry and the holistic patient care that is just as necessary for this.”
Fabian Hirsch

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Do you still have questions about our products and their application?
Simply arrange a telephone appointment with our experts.


As a manufacturer of medical devices, we apply strict quality criteria. Each of our products as well as the selection of components and partners are subject to the highest legal standards. In studies and in exchange with international experts, our scientists verify the effectiveness of our solutions and pass on their experience to our customers for optimal application.

botiss biomaterials – on the safe side based on experience


Sie fragen sich wie Sie den Vitamin-D-Test in Ihrer Praxis abrechnen können? Wir haben die wichtigsten Informationen für Sie zusammengestellt und stellen Ihnen diese gerne zur Verfügung.


The importance of vitamin D

Vitamin D is the generic term for a group of vitamins, also referred to as calciferols. Vitamin D is naturally present in a few foods, added to others, and available as a dietary supplement. It is also produced by exposure of the skin to ultraviolet light or sunlight. In foods and dietary supplements, vitamin D has two main forms, D2 and D3 that slightly differ in their structure but are easily absorbed from the body. Vitamin D2 is found in yeast, mushrooms, as well as plants, whereas vitamin D3 can be found in foods of animal origin, such as cod liver oil and oily fish.

The body forms vitamin D independently mainly via the skin – with the help of UV-B radiation transported via sunlight. For this, a sufficient regular stay outdoors is necessary. Diet contributes only a relatively small amount to the vitamin D supply, as only few foods naturally contain sufficient quantities of vitamin D. These include fatty sea fish, certain offal, edible mushrooms and eggs. In addition to these natural sources, vitamin D can also be supplied via dietary supplements (supplements) and foods appropriately enriched with vitamins, such as fruit juices. Additional/ detailed information about vitamin D sources can be found here.


Apart from genetic differences, age can be a contributing factor to develop vitamin D deficiency, not only because adults are likely to spend more time indoors but also due to a declined ability to synthetize vitamin D through the skin . Furthermore, as the gastrointestinal tract plays a key role in the conversion of vitamin D from its inactive to active form, people who suffer from gastrointestinal diseases are at risk of reduced levels of vitamin D . Obesity also hampers vitamin D levels, as excess body fat can sequester vitamin D making it less available for the body’s needs . 

The importance of an adequate vitamin D level is due to its vital role in the regulation of various physiological processes. Vitamin D is known to:

  • help the body absorb calcium – essential for healthy teeth and bones;
  • help to reduce the risk of periodontal disease; 
  • promote bone health and prevents osteoporosis; 
  • help to boost your immune system.

Additionally, a positive impact of vitamin D on mental health and its role in protection against diseases, including cardiovascular conditions, aut

On the one hand, vitamin D regulates the calcium level in the blood and thus strengthens the tooth enamel. On the other hand, it is responsible for the production of antimicrobial proteins in the form of cathelicidins which act against harmful bacteria, caries and periodontitis pathogens. If the body does not produce enough vitamin D, this can lead not only to weakened gums and, in the long term, periodontitis, but also to brittle tooth enamel and thus to an increased susceptibility to caries. Furthermore, vitamin D has a generally positive influence on bone growth and wound healing9.

Vitamin D test in the dental practice 

Low levels of vitamin D can lead to a deficiency, which has been linked to conditions such as osteoporosis, increased risk of fractures, weakened immune system and muscle weakness 5. Additionally to the impact of vitamin D on general health, deficiencies can also impact oral health, contributing to tooth loss, gum disease (periodontitis), and dental caries (cavities) 9. Also, accumulating evidence has demonstrated that vitamin D deficiency is associated with an increase in early implant failure. Thus, monitoring vitamin D levels  helps to diagnose a potential deficiency and allows proper supplementation for upcoming oral treatments with the aim to support better predictability of the outcomes, reduce complication rate and also to contribute to higher success rate.

In order to establish the initial vitamin D levels and potentially detect a deficiency, a test is recommended at the first consultation meeting. Furthermore, testing throughout the treatment can be carried out to monitor vitamin D levels and access the state of the patients. After the medical intervention testing can be performed to validate the vitamin D levels. Nevertheless, multiple testing appointments could be beneficial if supplementation has been prescribed to monitor the patient compliance and also to examine the need for dose adjustment.


The botissCARE Rapi-D test is easy and can be carried out quickly by following the instructions. 

These steps are necessary:

1.Painless blood collection from the fingertip
2. Transferring the sample into the buffer liquid
3. Drop the buffer liquid into the test cassette
4. Starting the botiss Cube Reader and reading the test result after 15 minutes

The complete test process takes a total of approx. 17-20 minutes.

A detailed demonstration of the botissCARE Rapi-D test can be found here. 


When performing the botissCARE Rapi-D, it is important to pay attention to the following steps:

  • The first droplet of blood should be removed before filling the blood collector 
  • Wait until a proper blood droplet (2-3 mm) is formed (to increase the blood flow: massage hand & finger slightly but do not squeeze excessively)
  • Ensure that the capillary is always completely filled with blood
  • Ensure that the blood is mixed thoroughly by swinging in semicircular motion
  • Ensure that exactly 3 blood droplets are placed on the test cassette

Please note that measurement time is essential, as variations in the incubation time may have influence on the generated results and may interfere with the test. Therefore, ensure that the timer is set for exactly 15 minutes.

Vitamin D levels are classically determined in the laboratory. However, the process of blood collection, sample transport to an external laboratory and transmission of the final results to the dentist can face various challenges and can often be a time consuming and expensive service. In comparison, the botissCARE Rapi-D test enables a fast and flexible use directly at the point-of-care to detect a vitamin D deficiency with great reliability, sensitivity and specificity. The botissCARE vitamin D test has proven its accuracy with a correlation coefficient of 98 % to liquid chromatography with tandem mass spectrometry, which has established itself as the gold standard in vitamin D diagnostics .

Vitamin D values are reported in different units of measurement. Commonly used are nmol/l (nanomoles per liter) and ng/ml (nanograms per milliliter). The nmol/l value is 2.5 times higher than the ng/ml value. The botissCARE cube reader gives the test result in the unit of measurement ng/ml. Vitamin D supplements use the unit of measurement I.E. (international unit). This unit stands for the effect and does not describe a substance quantity. 1 I.E. is equivalent to 0.025 μg. The vitamin D dosage recommendation is based on the daily dose in I.E.

botissCARE D3+K2 Spray

botissCARE D3+K2 Spray is a food supplement containing vitamin D that is recommended for adults who have been diagnosed with a vitamin D deficiency.It is important to check for any potential interactions with other medications your patient may be taking. The spray is not intended to replace a balanced diet or regular sunlight exposure. It is not recommended to be taken in combination with anticoagulants. Generally it is advised to review patients medication list, before recommending botissCARE D3+K2.  

botissCARE D3+K2 Spray I is a food supplement in the form of an oil based spray, which is composed of Vitamin D3 and Vitamin K2-MK7. As the most relevant forms for the human body, Vitamin D3 has the highest potency among the Vitamin D forms, while Vitamin K2 with the specific MK7 side chain is superior in terms of half-life compared to its other variants. botissCARE D3+K2 Spray offers a neutral taste and is  free of additives or colorants, vegetarian, gluten free, allergen free, lactose free, non-GMO.

In the human body, Vitamin D is a generic name of Vitamin D2 and D3. Vitamin D3 is formed in the human skin by exposure to the ultraviolet sunlight and can be obtained from diet and food supplements. Vitamin K2 occurs naturally in green vegetables and plant oils and one of its variants, which possesses a specific side chain length (MK7) and is also included in the spray has been shown to have a longer half-time life in plasma. Thus, it can reach the bone tissue more efficiently than other types of vitamin K and has a significant effect on bone health .

botissCARE D3+K2 Spray can be taken independently of the time of the day or meals. Food supplements are not a substitute for a balanced and varied diet as well as a healthy lifestyle.

botissCARE D3+K2 Spray Dosage 

Vitamin D intoxication, also known as vitamin D toxicity, can result from excessive intake of vitamin D from both dietary and non-dietary sources. Although it is uncommon to experience vitamin D toxicity solely from dietary sources, it can eventually develop if excessive quantities of supplements, high-dose medications (or a combination of both) are consumed over an extended period. The plasma concentration of 25(OH)D has to rise above 150 ng/ml to observe vitamin D intoxication .Therefore, this level should not be exceeded.

The spray mainly consists of Vitamin D3, Vitamin K2, coconut oil and olive oil. As fat-soluble vitamins, vitamin D and K play a major role in calcium metabolism and function synergistically in terms of bone health .  Osteocalcin is a calcium-binding protein responsible for the deposit of calcium in bones. Activation (carboxylation) of osteocalcin by vitamin K2 as a cofactor is critical for the integration of calcium into bone tissue .

The intra-oral spray technology is an alternative delivery mechanism which bypasses the digestive system, and is beneficial if digestive and malabsorption issues are present. Due to sublingual resorption, vitamins are resorbed faster and with better bioavailability. The botissCARE D3+K2 spray is compact and easy to carry and can be taken anytime as it does not require water or ingestion with meals.- Sprays are frequently more convenient to use, especially for people, who have difficulty with swallowing tablets. 

One spray in the mouth carries 1000 IU to the patient. Based on the guideline of the Endocrine Society, the recommended dose for adults (without any pre-existing conditions) to maintain optimal vitamin D levels should be 1500—2000 IU per day 13. 


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Product Management

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botiss biomaterials GmbH

Hauptstrasse 28
15806 Zossen / Germany
Tel.: +49 33769 / 88 41 985
Fax: +49 33769 / 88 41 986