The aim of the study was to evaluate the adhesion and proliferation of human fibroblasts on two native, non-cross-linked and one chemically cross-linked collagen membrane.
Human gingival fibroblasts were cultured on Jason® membrane, a human pericardium membrane (Regen) and a glutaraldehyde cross-linked bovine collagen membrane (BioMend® Extend™). Cell adhesion and survival were measured at different time points (24h, 48h, 72h, 7 days) using scanning electron microscopy and viability assays respectively.
The number of vital cells on the two non-cross-linked pericardial collagen membranes increased over time, while it decreased on the cross-linked membrane. The difference was statistically significant. Now difference was found between the two pericardium membranes. SEM-analysis revealed that fibroblasts adhered to all three membranes after 24h. After 7 days, however, cells were still attached to the native collagen membranes, whereas no cells were found on the cross-linked membrane.
The authors, Mohammad Reza Talebi Ardakani, concluded that the non-cross-linked collagen membranes equally support proliferation and adhesion of human gingival fibroblasts, whereas the chemically cross-linked collagen membrane did not.