Combination of an allogenic and a xenogenic bone substitute material with injectable platelet-rich fibrin – A comparative in vitro study

Dr. Peer Kämmerer et al.
Biomater. Appl. 2020 [Epub ahead of print]


The aim of this in vitro study was the comparison of allogeneic maxgraft® granules and xenogeneic BioOss® with and without injectable platelet-rich fibrin (i-PRF) on cell characteristics of human osteoblasts (HOB).

The bone grafting materials were incubated with HOB for 3, 7 and 10 days. HOB viability, migration, proliferation and differentiation (RT-PCR on alkaline phosphatase (AP), bone morphogenetic protein 2 (BMP-2) and osteonectin (OCN)) were measured and compared between the groups.

An increased viability, migration and proliferation was seen for the combination of maxgraft® granules and i-PRF at the 3 days time point. For viability and proliferation (days 7 and 10) and for migration (day 10), the combination of bone grafting materials with i-PRF showed higher values compared to the  bone grafting materials alone with maximum values for maxgraft® granules + i-PRF and minimum values for BioOss®.

Interestingly even maxgraft alone induced a significantly faster proliferation and increased cell viability of osteoblasts compared to BioOss alone.


  1. This in vitro study shows that maxgraft® granules with i-PRF demonstrate greater proliferation, differentiation, and cell-migration properties than Bio-Oss with i-PRF. Therefore, addition of i-PRF to allogeneic bone grafting material may influence osteoblast activity in vivo.
  2. Independently of iPRF, maxgraft® seems to have favorable effects on HOB compared to BioOss.

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